Hypoxia-regulated gene expression in fetal wound regeneration and adult wound repair

A Scheid1, R H Wenger1, H Christina1, I Camenisch1, A Ferenc1, U G Stauffer1, M Gassmann1, M Meuli1

Journal: PubMed

Date: 01/05/2002

1Department of Surgery, University Children's Hospital Zurich, Switzerland.

Prenatal diagnosis of a fetus with lumbar myelocystocele

N Kölble  1 , T A Huisman, T Stallmach, M Meuli, F Zen Ruffinen Imahorn, R Zimmermann

We present a case of a fetal lumbar myelocystocele, a rare congenital malformation, characterized by herniation of the central canal through a bony spina bifida. Routine ultrasound examination at 11 weeks' gestation by the primary obstetrician showed a suspicious cyst on the fetal back. Initially, the suspected diagnosis was a meningocele. After sonographic detection of newly developed fetal brain anomalies at 22 weeks' gestation the patient was referred to us. The enlarged cyst, which floated freely in the amniotic fluid, had a funnel-like appearance and was covered by a very thin layer of skin. With the help of ultrafast fetal magnetic resonance imaging the diagnosis of a fetal myelocystocele was made.

Journal: Ultrasound in Obstetrics & Gynecology (Volume 18, Issue 5, Pages 536-539)

Date: 01/11/2001

1 Unit of Perinatal Physiology, Department of Obstetrics, University Hospital, Zurich, Switzerland.

The Fetus with a Myelomeningocele (in: The Unborn Patient: The Art and Science of Fetal Therapy. 3rd ed. 2001, chapter 28:443-452. Authors: Michael R. Harrison, Mark I. Evans, N. Sc. Adzick. ISBN: 0-7216-8446-7)

Meuli M.

This popular reference returns in the 3rd Edition with an exciting new focus entirely committed to fetal therapy. Leading authorities from around the world provide comprehensive management strategies for several dozen diseases that can now be detected in utero. Each disease is covered in its own chapter providing all of the exciting new options in prenatal and postnatal treatment. Coverage includes updated information on pathophysiology and the technical aspects of treatment. This content also provides excellent teaching material for families considering or undergoing treatment.

Journal: Saunders: The Unborn Patient

Date: 01/01/2001

Assessment of sensory function in neonatal sheep with somatosensory evoked potentials: methodology and normative data

C D Yingling  1 , C Meuli-Simmen, M Meuli, G B Timmel, N S Adzick, M Harrison

Fetal sheep are increasingly used as animal models for fetal surgical interventions such as repair of myelomeningocele. Since behavioral observations cannot provide objective information about preservation of sensory function, we have developed a technique for reliably recording somatosensory evoked potentials in neonatal sheep. We determined anatomic criteria for placement of recording electrodes over the somatosensory cortex using external landmarks, and recorded normative data for both ulnar and posterior tibial nerve stimulation in a series of normal neonatal sheep. The methodology and normative data are presented in this report; a companion paper demonstrates the utilization of this technique in a variety of experimental fetal interventions.

Journal: Pediatric Surgery International 15, 530-534 (1999)

Date: 01/11/1999

1 Department of Neurological Surgery, University of California, San Francisco, USA.

Experimental fetal neurosurgery: effects of in-utero manipulations on somatosensory evoked potentials

C D Yingling  1 , C Meuli-Simmen, M Meuli, G B Timmel, M Harrison, N S Adzick

Somatosensory evoked potentials (SEP) were used to objectively evaluate sensory function in neonatal sheep after experimental fetal surgery. Posterior tibial (PTN) and ulnar (UN) nerves were stimulated electrically and averaged SEP were recorded from scalp electrodes placed over the somatosensory cortex. Animals with experimentally-created myelomeningocele (MMC) showed no SEP to PTN stimulation, but normal SEP to UN stimulation. In-utero repair of the MMC resulted in preservation of neurologic function and normal PTN SEP. In-utero thoracic spinal-cord transection resulted in no regeneration, and no SEP to PTN stimulation. In-utero unilateral transection of the sciatic nerve, even with attempted repair, resulted in little or no regeneration and absent or grossly abnormal PTN SEP from the affected side. In summary, the SEP technique provides valuable information concerning preservation of sensory function in a variety of experimentally created neurologic abnormalities and can aid in functional evaluation of experimental therapeutic fetal interventions.

Journal: Pediatric Surgery International 15, 535-539 (1999)

Date: 01/11/1999

1 Department of Neurological Surgery, University of California, San Francisco, USA.